Molecular oncology test menu

Disease category

Molecular genetic target

Corresponding translocation (where present)

Method

Lymphoid neoplasm

IGH rearrangement (MBH) 

 

B cell clonality analysis by PCR and GeneScan

IGK gene rearrangement (MBK) 

IGL gene rearrangement (MBL)

TCRG gene rearrangement (MTG)

 

T cell clonality analysis by PCR and GeneScan

TCRB gene rearrangement (MTB) 

TCRD gene rearrangement (MTD)

MALT lymphoma

API2-MALT1 (MMAPI2) 

t(11;18)

RT-PCR

Follicular or diffuse large B-cell lymphoma

BCL2-IGH (MHBCL2)

t(14;18)

RT-PCR

Mantle cell lymphoma

BCL1-IGH (MHBCL1)

t(11;14)

RT-PCR

Colorectal cancer/ non-small cell lung cancer

KRAS

 

Sanger sequencing/ HRM/ NGS

Non-small cell lung cancer

EGFR

 

Sanger sequencing/ NGS

Gastrointestinal stromal tumour

KIT and PDGFRA

 

PCR/ sequencing/ NGS

Melanoma/ colorectal cancer

BRAF

 

Sanger sequencing/ HRM/ NGS

Lymphoid neoplasms

IGH, IGK, IGL

 

FISH

Anaplastic large cell lymphoma

ALK

 

Mantle cell lymphoma

BCL1, BCL1-IGH

t(11;14)

Follicular/diffuse large B-cell lymphoma

BCL2, BCL2-IGH

t(14;18)

Diffuse large B cell/follicular lymphoma

BCL6

 

MALT lymphoma

MALT1, IGH-MALT1, API2-MALT1

t(14;18), t(11;18)

Burkitt/diffuse large B-cell lymphoma

MYC, IGH-MYC

t(8:14)

Neuroblastoma

MYCN, TOP2A

 

1p36

 

Ewings sarcoma

EWSR1

 

Rhabdomyosarcoma

FOXO1, PAX3

 

PAX7

 

Synovial sarcoma

SS18               

 

Dermatofibrosarcoma protuberans

PDGFB

 

Anaplastic oligodendroglioma

1p36/19q13

 

Pilocytic astrocytoma

KIAA1549-BRAF

t(1;7)

Non-small cell lung cancer

ALK, EML4-ALK

 

Tissue identification

CEP X/Y

 

Other services

STR genotyping to test tissue identity

 

PCR and GeneScan

Sectioning (macro/micro) of paraffin-embedded tissue, DNA preparation

 

Microtomy