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Bone marrow trephine

 

Unfixed

 

Unfixed bone marrow trephine biopsies should be sent in the formalin. Specimen should be immersed in 6 –10 times the volume of neutral buffered formalin (10%) in an appropriately sized container and the lid secured properly. The pot should be labelled with a formalin hazard label.

    Bone marrow trephine

     

    Fixed

     

    Fixed and stained sections
    or
    Block
    or
    Block plus 20 consecutive serial sections

    • 2 – 4 micrometers thin with minimal trimming
    • submitted as three H&E stained slides at level 1, 10 and 20 with remaining (17) unstained slides for further IHC/ retic

    Lymph node core biopsy

     

    Unfixed

     

    Unfixed lymph node samples can be sent either FRESH or in formalin.

    Lymph node cores, where lymphoma is suspected, the sample should be sent FRESH in an appropriately sized, labelled, dry container. Arrange in advance with the laboratory, telephone ext.58212 (CUH only) and arrange for the specimen to be transported to Histopathology immediately by a theatre porter. Routine specimens should be sent in formalin, immersed in six to ten times the volume of neutral buffered formalin (10%) in an appropriately sized container and the lid secured properly. The pot should be labelled with a formalin hazard label.

    Lymph node core biopsy

     

    Fixed

     

    Block
    or
    Block plus 20 consecutive serial sections (2-4 micro meters thin) with minimal trimming. Submitted as three H&E stained slides at level 1, 10 and 20 with remaining (17) unstained slides for further IHC/ retic.


    Lymph node excision

     

    Unfixed

     

    Unfixed lymph node samples can be sent either FRESH or in formalin.

    Lymph node sample, where lymphoma is suspected, the sample should be sent FRESH in an appropriately sized, labelled, dry container. Arrange in advance with the laboratory, telephone ext.58212 (CUH only) and arrange for the specimen to be transported to Histopathology immediately by a theatre porter.

    Routine specimens should be sent in formalin, immersed in six to ten times the volume of neutral buffered formalin (10%) in an appropriately sized container and the lid secured properly. The pot should be labelled with a formalin hazard label.

    Lymph node excision

     

    Fixed

     

    Submit block(s)
    or
    Single H&E stained slide from each block(s) plus block(s)
    or
    Single H&E stained slide from each block(s) plus 20 unstained slides from each block(s)


    Splenic biopsy

     

    Unfixed

     

    Unfixed splenic core biopsy samples can be sent either FRESH or in formalin.
    Splenic cores, where lymphoma is suspected, the sample should be sent FRESH in an appropriately sized, labelled, dry container. Arrange in advance with the laboratory, telephone ext.58212 (CUH only) and arrange for the specimen to be transported to Histopathology immediately by a theatre porter.

    Routine specimens should be sent in formalin, immersed in six to ten times the volume of neutral buffered formalin (10%) in an appropriately sized container and the lid secured properly. The pot should be labelled with a formalin hazard label.

    Splenic biopsy

     

    Fixed

     

    Block
    or
    Block plus 20 consecutive serial sections (2-4 micro meters thin) with minimal trimming. Submitted as three H&E stained slides at level 1, 10 and 20 with remaining (17) unstained slides for further IHC/retic.


    Cytology Samples

     

    Unfixed

     

    For pleural and ascitic fluid samples, ideally, at least 50mls should be sent in plain universal container without delay to the Diagnostic Cytology Laboratory on level 5 at Addenbrooke’s Hospital. A separate sample should be sent to HODS if flow cytometry is required.