Spleen core biopsy

Unfixed sample

Unfixed splenic core biopsy samples can be sent either FRESH or in formalin. Splenic cores, where lymphoma is suspected, the sample should be sent FRESH in an appropriately sized, labelled, dry container. Arrange in advance with the laboratory, telephone ext.58212 and arrange for the specimen to be transported to Histopathology immediately by a theatre porter. Routine specimens should be sent in formalin, immersed in six to ten times the volume of neutral buffered formalin (10%) in an appropriately sized container and the lid secured properly. The pot should be labelled with a formalin hazard label.

Fixed and stained sections

Submit block 


  • 20 consecutive serial sections (2-4 micro meters thin) with minimal trimming and submitted as following:
  • Three H&E stained slides at level 1, 10 and 20

+ Rest (17) unstained slides for further IHC

+ Bloc 

Sample reviewed at HODS, agreement or amendment of local report without/ with IHC if needed and report added to the system.